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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- GTX20265 - Provider product page
- Provider
- GeneTex
- Product name
- SPRY4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-Sprouty-4 antibody. Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 ?g per lane.Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4¢XC.Secondary antibody: IRDye800? rabbit secondary antibody at 1:5,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4¢XC.Predicted/Observed size: a doublet band ~35 kDa corresponding to human Sprouty-4. Other band(s): none.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: Sprouty-4 antibody at 2.5 ?g/ml for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: Sprouty-4 is cytoplasmic.Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: Sprouty-4 antibody at 2.5 ?g/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: Sprouty-4 is cytoplasmic.Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.
