PA1-28838

antibody from Invitrogen Antibodies

Targeting: GLI2 HPE9, THP1, THP2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-28838

Provider

Invitrogen Antibodies

Product name

GLI2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-28838 detects Gli2 from mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

50 µg

Concentration

1.02 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Suppressor of fused controls cerebellum granule cell proliferation by suppressing Fgf8 and spatially regulating Gli proteins.

Jiwani T, Kim JJ, Rosenblum ND
Development (Cambridge, England) 2020 Feb 3;147(3)

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Novel mTORC1 Mechanism Suggests Therapeutic Targets for COMPopathies.

Posey KL, Coustry F, Veerisetty AC, Hossain MG, Gambello MJ, Hecht JT
The American journal of pathology 2019 Jan;189(1):132-146

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Gli2 in mouse brain whole cell lysate using a Gli2 polyclonal antibody (Product # PA1-28838) at a dilution of 1:750. Results show detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in lane 1. Molecular weight markers are shown (M) using the 700 nm channel (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot using GLI2 Polyclonal Antibody (Product # PA1-28838) shows detection of a predominant band at 190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). 25 µg of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:750. Incubation was at room temperature for 2 h followed by washes and reaction with a 1:10,000 dilution of a Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown (M) using the 700 nm channel (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GLI2 was performed in several formalin-fixed and paraffin embedded mouse brain using GLI2 Polyclonal Antibody (Product # PA1-28838) at 10 µg/mL. Moderate to strong staining was seen on many tissues with low background staining. This image shows Gli-2 staining of mouse brain.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GLI2 was performed in several formalin-fixed and paraffin embedded mouse testis using GLI2 Polyclonal Antibody (Product # PA1-28838) at 10 µg/mL. Moderate to strong staining was seen on many tissues with low background staining. This image shows Gli-2 staining of mouse testis.