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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB600-874 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-874, RRID:AB_10001953
- Product name
- Rabbit Polyclonal GLI-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB600-874 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Gli2 mediates the development of castration‑resistant prostate cancer.
Direct repression of anoctamin 1 (ANO1) gene transcription by Gli proteins.
Differential effects of GLI2 and GLI3 in regulating cervical cancer malignancy in vitro and in vivo.
Gli Transcription Factors Mediate the Oncogenic Transformation of Prostate Basal Cells Induced by a Kras-Androgen Receptor Axis.
Modulation of the IL-6 Receptor α Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenström Macroglobulinemia Cells.
Autophagy is related to the hedgehog signaling pathway in human gastric adenocarcinoma: prognostic significance of Beclin-1 and Gli2 expression in human gastric adenocarcinoma.
Xia L, Bouamar H, Gu X, Zeballos C, Qin T, Wang B, Zhou Y, Wang Y, Yang J, Zhu H, Zhang W, Houghton PJ, Sun LZ
International journal of oncology 2020 Jul;57(1):100-112
International journal of oncology 2020 Jul;57(1):100-112
Direct repression of anoctamin 1 (ANO1) gene transcription by Gli proteins.
Mazzone A, Gibbons SJ, Eisenman ST, Strege PR, Zheng T, D'Amato M, Ordog T, Fernandez-Zapico ME, Farrugia G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 May;33(5):6632-6642
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 May;33(5):6632-6642
Differential effects of GLI2 and GLI3 in regulating cervical cancer malignancy in vitro and in vivo.
Zhu H, Xia L, Shen Q, Zhao M, Gu X, Bouamar H, Wang B, Sun LZ, Zhu X
Laboratory investigation; a journal of technical methods and pathology 2018 Nov;98(11):1384-1396
Laboratory investigation; a journal of technical methods and pathology 2018 Nov;98(11):1384-1396
Gli Transcription Factors Mediate the Oncogenic Transformation of Prostate Basal Cells Induced by a Kras-Androgen Receptor Axis.
Wu M, Ingram L, Tolosa EJ, Vera RE, Li Q, Kim S, Ma Y, Spyropoulos DD, Beharry Z, Huang J, Fernandez-Zapico ME, Cai H
The Journal of biological chemistry 2016 Dec 2;291(49):25749-25760
The Journal of biological chemistry 2016 Dec 2;291(49):25749-25760
Modulation of the IL-6 Receptor α Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenström Macroglobulinemia Cells.
Jackson DA, Smith TD, Amarsaikhan N, Han W, Neil MS, Boi SK, Vrabel AM, Tolosa EJ, Almada LL, Fernandez-Zapico ME, Elsawa SF
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 15;195(6):2908-16
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 15;195(6):2908-16
Autophagy is related to the hedgehog signaling pathway in human gastric adenocarcinoma: prognostic significance of Beclin-1 and Gli2 expression in human gastric adenocarcinoma.
Won KY, Kim GY, Lim SJ, Sung JY, Kim YW, Park YK, Lee J, Choi HS
Pathology, research and practice 2015 Apr;211(4):308-15
Pathology, research and practice 2015 Apr;211(4):308-15
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GLI-2 Antibody [NB600-874] - Analysis of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GLI-2 Antibody [NB600-874] - Staining of tumor cells in human breast tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): GLI-2 Antibody [NB600-874] - intracellular staining for GLI-2 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-2 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.
