Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-03269 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HDAC6 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Epigenetic effects of chromatin remodeling agents on organotypic cultures.
Sirchia SM, Faversani A, Rovina D, Russo MV, Paganini L, Savi F, Augello C, Rosso L, Del Gobbo A, Tabano S, Bosari S, Miozzo M
Epigenomics 2016 Mar;8(3):341-58
Epigenomics 2016 Mar;8(3):341-58
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC6 Antibody (1B3) [NBP2-03269] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC6 Antibody (1B3) [NBP2-03269] Analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human Kidney tissue using anti-HDAC6 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human liver tissue using anti-HDAC6 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC6 Antibody (1B3) [NBP2-03269] - Staining of paraffin-embedded Human pancreas tissue using anti-HDAC6 mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HDAC6 Antibody (1B3) [NBP2-03269] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC6 antibody, and then analyzed by flow cytometry.