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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-1983 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1983, RRID:AB_10001339
- Product name
- Mouse Monoclonal CaM Kinase II alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.
- Reactivity
- Human, Mouse, Rat, Bovine, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Effects of antiepileptic drugs in a new TSC/mTOR-dependent epilepsy mouse model.
The Retinal Ganglion Cell Transportome Identifies Proteins Transported to Axons and Presynaptic Compartments in the Visual System In Vivo.
Traumatic Stress Produces Distinct Activations of GABAergic and Glutamatergic Neurons in Amygdala.
Role of the visual experience-dependent nascent proteome in neuronal plasticity.
Satb2 Ablation Impairs Hippocampus-Based Long-Term Spatial Memory and Short-Term Working Memory and Immediate Early Genes (IEGs)-Mediated Hippocampal Synaptic Plasticity.
The glutamate receptor GluN2 subunit regulates synaptic trafficking of AMPA receptors in the neonatal mouse brain.
D-serine and serine racemase are localized to neurons in the adult mouse and human forebrain.
Bidirectional influence of sodium channel activation on NMDA receptor-dependent cerebrocortical neuron structural plasticity.
Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells.
Koene LMC, van Grondelle SE, Proietti Onori M, Wallaard I, Kooijman NHRM, van Oort A, Schreiber J, Elgersma Y
Annals of clinical and translational neurology 2019 Jul;6(7):1273-1291
Annals of clinical and translational neurology 2019 Jul;6(7):1273-1291
The Retinal Ganglion Cell Transportome Identifies Proteins Transported to Axons and Presynaptic Compartments in the Visual System In Vivo.
Schiapparelli LM, Shah SH, Ma Y, McClatchy DB, Sharma P, Li J, Yates JR 3rd, Goldberg JL, Cline HT
Cell reports 2019 Aug 13;28(7):1935-1947.e5
Cell reports 2019 Aug 13;28(7):1935-1947.e5
Traumatic Stress Produces Distinct Activations of GABAergic and Glutamatergic Neurons in Amygdala.
Fang Q, Li Z, Huang GD, Zhang HH, Chen YY, Zhang LB, Ding ZB, Shi J, Lu L, Yang JL
Frontiers in neuroscience 2018;12:387
Frontiers in neuroscience 2018;12:387
Role of the visual experience-dependent nascent proteome in neuronal plasticity.
Liu HH, McClatchy DB, Schiapparelli L, Shen W, Yates JR 3rd, Cline HT
eLife 2018 Feb 7;7
eLife 2018 Feb 7;7
Satb2 Ablation Impairs Hippocampus-Based Long-Term Spatial Memory and Short-Term Working Memory and Immediate Early Genes (IEGs)-Mediated Hippocampal Synaptic Plasticity.
Li Y, You QL, Zhang SR, Huang WY, Zou WJ, Jie W, Li SJ, Liu JH, Lv CY, Cong J, Hu YY, Gao TM, Li JM
Molecular neurobiology 2017 Apr 18;
Molecular neurobiology 2017 Apr 18;
The glutamate receptor GluN2 subunit regulates synaptic trafficking of AMPA receptors in the neonatal mouse brain.
Hamada S, Ogawa I, Yamasaki M, Kiyama Y, Kassai H, Watabe AM, Nakao K, Aiba A, Watanabe M, Manabe T
The European journal of neuroscience 2014 Oct;40(8):3136-46
The European journal of neuroscience 2014 Oct;40(8):3136-46
D-serine and serine racemase are localized to neurons in the adult mouse and human forebrain.
Balu DT, Takagi S, Puhl MD, Benneyworth MA, Coyle JT
Cellular and molecular neurobiology 2014 Apr;34(3):419-35
Cellular and molecular neurobiology 2014 Apr;34(3):419-35
Bidirectional influence of sodium channel activation on NMDA receptor-dependent cerebrocortical neuron structural plasticity.
George J, Baden DG, Gerwick WH, Murray TF
Proceedings of the National Academy of Sciences of the United States of America 2012 Nov 27;109(48):19840-5
Proceedings of the National Academy of Sciences of the United States of America 2012 Nov 27;109(48):19840-5
Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells.
Feng T, Szabo E, Dziak E, Opas M
Stem cell reviews and reports 2010 Mar;6(1):74-85
Stem cell reviews and reports 2010 Mar;6(1):74-85
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 . Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:10000 for 12 hours at 4 degrees C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.
