STJ97419

antibody from St John's Laboratory

Targeting: CYCS CYC, HCS

Immunocytochemistry

Antibody data

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Validation data

Product number: STJ97419 - Provider product page
Provider: St John's Laboratory
Product name: Anti-CYCS antibody [4B10] (STJ97419)
Antibody type: Monoclonal
Description: Mouse monoclonal antibody anti-Cytochrome C is suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry research applications.
Reactivity: Human, Mouse, Rat, Chicken/Avian
Host: Mouse
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Handling: NA

CYCS protein structure - STJ97419 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of chicken cell lysis using Antibody diluted at 1:1000
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) Mouse Liver Tissue, 5) Rat Liver Tissue, 6) Rat Kidney Tissue using CYCS monoclonal antibody.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of lysates from 1) Hela, 2) 293T, 3) 3T3, cells, ï¼Greenï¼ primary antibody was diluted at 1:1000, 4Â°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37Â°C 1hour. ï¼Redï¼ GAPDH Polyclonal Antibody (cat: (STJ97090) antibody was diluted at 1:5000 as loading control, 4Â°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37Â°C 1hour.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Rat-liver tissue. 1, CYCS monoclonal antibody (4B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Human-liver-cancer tissue. 1, CYCS monoclonal antibody (4B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Mouse-spleen tissue. 1, CYCS monoclonal antibody (4B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Hela cell. 1, c-Myc Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using CYCS monoclonal antibody.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, CYCS monoclonal antibody (4B10) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, CYCS monoclonal antibody (4B10) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, CYCS monoclonal antibody (4B10) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA