Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 54205-RBM6-P0 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS, 3128R)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Drosophila
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- CYCS, 3128R
- Vial size
- 20 µg
- Concentration
- 200 µg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of human heart tissue lysate using Cytochrome C Recombinant Monoclonal Antibody (Product # 54205-RBM6-P1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of PFA fixed HeLa cells using Cytochrome C Recombinant Monoclonal Antibody (Product # 54205-RBM6-P1) followed by goat anti-rabbit IgG-CF488 (Green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human liver using Cytochrome C Recombinant Monoclonal Antibody (Product # 54205-RBM6-P1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of PFA fixed HeLa cells using Cytochrome C Recombinant Monoclonal Antibody (Product # 54205-RBM6-P1) followed by goat anti-rabbit IgG-CF488 (Blue); Goat anti-mouse IgG-CF488 is Control (Red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDS-PAGE Analyis Purified Cytochrome C Recombinant Monoclonal Antibody (Product # 54205-RBM6-P1). Confirmation of Purity and Integrity of Antibody.
