H00008493-M01

antibody from Abnova Corporation

Targeting: PPM1D PP2C-DELTA, Wip1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008493-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008493-M01, RRID:AB_606821

Product name

PPM1D monoclonal antibody (M01), clone 4D1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PPM1D.

Antigen sequence

IGLVPTNSTNTVMDQKNLKMSTPGQMKAQEIERTP
PTNFKRTLEESNSGPLMKKHRRNGLSRSSGAQPAS
LPTTSQRKNSVKLTMRRRLRGQKKIGNPLLHQHRK
TVCVC


Isotype

IgG

Antibody clone number

4D1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PPM1D expression in transfected 293T cell line by PPM1D monoclonal antibody (M01), clone 4D1.Lane 1: PPM1D transfected lysate(66.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PPM1D is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol