692602

antibody from BioLegend

Targeting: HGF DFNB39, F-TCF, HGFB, HPTA, SF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

692602

Provider

BioLegend

Proper citation

BioLegend Cat#692602, RRID:AB_2632766

Product name

Purified anti-HGF

Antibody type

Monoclonal

Description

HGF (Hepatocyte growth factor) is a multifunctional cytokine that plays important roles in cell proliferation, survival, motility and morphogenesis. HGF protects the epithelium, neurons and cardiomyocytes during organ diseases; this protective effect is mediated through anti-apoptotic signals via inhibition of caspase-3 activity or induction of anti-apoptotic molecules. Secreted by cells of mesenchymal origin, HGF is the specific ligand for the tyrosine-kinase receptor c-MET (cellular mesenchymal-epithelial transition), also called MET, which is expressed in different types of epithelial, endothelial and hematopoietic progenitor cells. Aberrantly activated MET/HGF signaling correlates with tumorigenesis and metastasis, and is regarded as a target for novel anti-cancer treatments.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

A15061E

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (30 µg protein) from U87-MG (lane 1), THP-1 untreated (lane 2), THP-1 treated with PMA for three days and LPS for one day (lane 3), Raw264.7 (lane 4) and 5 ng recombinant human HGF (lane 5) were resolved by 4-12% Bis-Tris gel electrophoresis under non-reducing condition, transferred to nitrocellulose, and probed with purified anti-HGF (clone A15061E) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.

Conjugate

Unconjugated