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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB4540 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4540, RRID:AB_1579144
- Product name
- PRKCI polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRKCI.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Protein kinase C (PKC) betaII induces cell invasion through a Ras/Mek-, PKC iota/Rac 1-dependent signaling pathway.
Rab2 interacts directly with atypical protein kinase C (aPKC) iota/lambda and inhibits aPKCiota/lambda-dependent glyceraldehyde-3-phosphate dehydrogenase phosphorylation.
Rapid backbone 1H, 13C, and 15N assignment of the V1 domain of human PKC iota using the new program IBIS.
Zhang J, Anastasiadis PZ, Liu Y, Thompson EA, Fields AP
The Journal of biological chemistry 2004 May 21;279(21):22118-23
The Journal of biological chemistry 2004 May 21;279(21):22118-23
Rab2 interacts directly with atypical protein kinase C (aPKC) iota/lambda and inhibits aPKCiota/lambda-dependent glyceraldehyde-3-phosphate dehydrogenase phosphorylation.
Tisdale EJ
The Journal of biological chemistry 2003 Dec 26;278(52):52524-30
The Journal of biological chemistry 2003 Dec 26;278(52):52524-30
Rapid backbone 1H, 13C, and 15N assignment of the V1 domain of human PKC iota using the new program IBIS.
Roehrl MH, Hyberts SG, Sun ZY, Fields AP, Wagner G
Journal of biomolecular NMR 2003 Aug;26(4):373-4
Journal of biomolecular NMR 2003 Aug;26(4):373-4
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRKCI polyclonal antibody (Cat # PAB4540) in mouse lung lysate . PRKCI (arrow) was detected using purified PRKCI polyclonal antibody (Cat # PAB4540) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRKCI (arrow) using rabbit PRKCI polyclonal antibody (Cat # PAB4540). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCI gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PRKCI polyclonal antibody (Cat # PAB4540) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
