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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005584-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005584-M01, RRID:AB_547080
- Product name
- Mouse Monoclonal PKC iota Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PRKCI - protein kinase C, iota
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
Bambang IF, Lee YK, Richardson DR, Zhang D
Oncogene 2013 Mar 7;32(10):1240-51
Oncogene 2013 Mar 7;32(10):1240-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKC iota Antibody (1G11) [H00005584-M01] - Analysis of PRKCI over-expressed 293 cell line, cotransfected with PRKCI Validated Chimera RNAi ( Cat # H00005584-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCI monoclonal antibody (M01) clone 1G11 (Cat # H00005584-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKC iota Antibody (1G11) [H00005584-M01] - Analysis of PRKCI expression in transfected 293T cell line by PRKCI monoclonal antibody (M01), clone 1G11.Lane 1: PRKCI transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PKC iota Antibody (1G11) [H00005584-M01] - Detection limit for recombinant GST tagged PRKCI is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PKC iota Antibody (1G11) [H00005584-M01] - Analysis of monoclonal antibody to PRKCI on HeLa cell . Antibody concentration 10 ug/ml.
