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Learn13883-1-AP
antibody from Invitrogen Antibodies
Targeting: PRKCI
DXS1179E, PKCI
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 13883-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKC iota Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SSLGLQDFD LLRVIGRGSY AKVLLVRLKK TDRIYAMKVV KKELVNDDED IDWVQTEKHV FEQASNHPFL VGLHSCFQTE SRLFFVIEYV NGGDLMFHMQ RQRKLPEEHA RFYSAEISLA LNYLHERGII YRDLKLDNVL LDSEGHIKLT DYGMCKEGLR PGDTTSTFCG TPNYIAPEIL RGEDYGFSVD WWALGVLMFE MMAGRSPFDI VGSSDNPDQN TEDYLFQVIL EKQIRIPRSM SVKAASVLKS FLNKDPKERL GCLPQTGFAD IQGHPFFRNV DWDMMEQKQV VPPFKPNISG EFGLDNFDSQ FTNERVQLTP DDDDIVRKID QSEFEGFEYI NPLLMSAEEC V (238-587 aa encoded by BC022016)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 13883-1-AP (PKC iota antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13883-1-AP (PKC iota antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 13883-1-AP (PKC iota antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13883-1-AP (PKC iota antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13883-1-AP (PKC iota antibody at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human PKC Iota (Product # 13883-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PKC iota (IP:13883-1-AP, 3ug; Detection:13883-1-AP 1:500) with HeLa cells lysate 2500ug.
