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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- MAB931-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse BACE-1 Ectodomain Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse BACE-1 in direct ELISAs and human BACE-1 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BACE-2, recombinant mouse (rm) BACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P56817- Isotype
- IgG
- Antibody clone number
- 137612
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Deficiency of Neuronal p38α MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1.
Overexpression of the IGF-II/M6P receptor in mouse fibroblast cell lines differentially alters expression profiles of genes involved in Alzheimer's disease-related pathology.
High activities of BACE1 in brains with mild cognitive impairment.
BACE1 and BACE2 enzymatic activities in Alzheimer's disease.
Schnöder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y
The Journal of biological chemistry 2016 Jan 29;291(5):2067-79
The Journal of biological chemistry 2016 Jan 29;291(5):2067-79
Overexpression of the IGF-II/M6P receptor in mouse fibroblast cell lines differentially alters expression profiles of genes involved in Alzheimer's disease-related pathology.
Wang Y, Thinakaran G, Kar S
PloS one 2014;9(5):e98057
PloS one 2014;9(5):e98057
High activities of BACE1 in brains with mild cognitive impairment.
Cheng X, He P, Lee T, Yao H, Li R, Shen Y
The American journal of pathology 2014 Jan;184(1):141-7
The American journal of pathology 2014 Jan;184(1):141-7
BACE1 and BACE2 enzymatic activities in Alzheimer's disease.
Ahmed RR, Holler CJ, Webb RL, Li F, Beckett TL, Murphy MP
Journal of neurochemistry 2010 Feb;112(4):1045-53
Journal of neurochemistry 2010 Feb;112(4):1045-53
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human BACE-1 by Western Blot. Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse BACE-1 Ectodomain Monoclonal Antibody (Catalog # MAB931) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for BACE-1 at approximately 60 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
