AF-243-NA

antibody from Novus Biologicals

Targeting: TGFB3 ARVD, ARVD1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-243-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal TGF-beta 3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects TGF-beta 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 25% cross-reactivity with recombinant amphibian TGF-beta 5 is observed, less than 10% cross-reactivity with TGF-beta 1, TGF-beta 1.2, and TGF-beta 2 is observed, and less than 5% cross-reactivity with recombinant human LAP (TGF-beta 1) is observed.

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor β (TGFβ)-, epidermal growth factor (EGF)-, and nuclear factor κB (NFκB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion.

Kang I, Yoon DW, Braun KR, Wight TN
The Journal of biological chemistry 2014 May 30;289(22):15393-404

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Transforming growth factor Beta 3 is required for excisional wound repair in vivo.

Le M, Naridze R, Morrison J, Biggs LC, Rhea L, Schutte BC, Kaartinen V, Dunnwald M
PloS one 2012;7(10):e48040

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Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.

Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 May;31(5):516-22

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Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure.

Neurohr C, Nishimura SL, Sheppard D
American journal of respiratory cell and molecular biology 2006 Aug;35(2):252-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of TGF-beta 3 by Western Blot. Western blot shows lysates of Saos-2 human osteosarcoma cell line, L-929 mouse fibroblast cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-TGF-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF-beta 3 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human TGF-beta 3 by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line and Saos-2 human osteosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for TGF-beta 3 at approximately 67 kDa (as indicated) using 10 µg/mL of Goat Anti-TGF-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

TGF-beta 3 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 3 Antibody. Recombinant Human TGF-beta 3 (Catalog # 243-B3) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of TGF-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA). The ND₅₀ is typically 0.01-0.05 µg/mL.