PA5-95999

antibody from Invitrogen Antibodies

Targeting: U2AF2 U2AF65

Immunoprecipitation

Antibody data

Submit

Validation data

Product number: PA5-95999 - Provider product page
Provider: Invitrogen Antibodies
Product name: U2AF2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: NQDKNFAFLE FRSVDETTQA MAFDGIIFQG QSLKIRRPHD YQPLPGMSEN PSVYVPGVVS TVVPDSAHKL FIGGLPNYLN DDQVKELLTS FGPLKAFNLV KDSATGLSKG YAFCEYVDIN VTDQAIAGLN GMQLGDKKLL VQRASVGAKN ATLSTINQTP VTLQVPGLMS SQVQMGGHPT EVLCLMNMVL PEELLDDEEY EEIVEDVRDE CSKYGLVKSI EIPRPVDGVE VPGCGKIFVE FTSVFDCQKA MQGLTGRKFA NRVVVTKYCD PDSYHRRDFW; Positive Samples: Jurkat, SW620, Mouse spleen; Cellular Location: Nucleus
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.61 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

U2AF2 protein structure - PA5-95999 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of extracts of various cell lines, using U2AF2 Polyclonal antibody (Product # PA5-95999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of U2AF2 in extracts of various cell lines using U2AF2 Polyclonal Antibody (Product # PA5-95999) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extract (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), A-431 (Lane 3), and Jurkat (Lane 4). The blot was probed with Anti-U2AF2 Polyclonal Antibody (Product # PA5-95999, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 55 kDa band corresponding to U2AF2 was detected in cell lines and tissue tested along with uncharacterized bands at ~42 kDa and ~82 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of U2AF2 in extracts of various cell lines using U2AF2 Polyclonal Antibody (Product # PA5-95999) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.