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- Antibody Data
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- References [12]
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- Blocking/Neutralizing [1]
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- Product number
- MAB126-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Fas Ligand/TNFSF6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Fas Ligand/TNFSF6 in ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P48023- Isotype
- IgG
- Antibody clone number
- 100419
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer.
Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells.
Granulocytic myeloid-derived suppressor cells from human cord blood modulate T-helper cell response towards an anti-inflammatory phenotype.
Interleukin-10-producing LAG3+ regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis.
Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways.
Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice.
The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice.
Reciprocal complementation of the tumoricidal effects of radiation and natural killer cells.
Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins.
KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
Inhibition of p38 mitogen-activated protein kinase unmasks a CD30-triggered apoptotic pathway in anaplastic large cell lymphoma cells.
A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
Kim M, Pyo S, Kang CH, Lee CO, Lee HK, Choi SU, Park CH
PloS one 2018;13(6):e0198347
PloS one 2018;13(6):e0198347
Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells.
Idelson M, Alper R, Obolensky A, Yachimovich-Cohen N, Rachmilewitz J, Ejzenberg A, Beider E, Banin E, Reubinoff B
Stem cell reports 2018 Sep 11;11(3):681-695
Stem cell reports 2018 Sep 11;11(3):681-695
Granulocytic myeloid-derived suppressor cells from human cord blood modulate T-helper cell response towards an anti-inflammatory phenotype.
Köstlin N, Vogelmann M, Spring B, Schwarz J, Feucht J, Härtel C, Orlikowsky TW, Poets CF, Gille C
Immunology 2017 Sep;152(1):89-101
Immunology 2017 Sep;152(1):89-101
Interleukin-10-producing LAG3+ regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis.
Nakachi S, Sumitomo S, Tsuchida Y, Tsuchiya H, Kono M, Kato R, Sakurai K, Hanata N, Nagafuchi Y, Tateishi S, Kanda H, Okamura T, Yamamoto K, Fujio K
Arthritis research & therapy 2017 May 16;19(1):97
Arthritis research & therapy 2017 May 16;19(1):97
Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways.
Tanzer MC, Khan N, Rickard JA, Etemadi N, Lalaoui N, Spall SK, Hildebrand JM, Segal D, Miasari M, Chau D, Wong WL, McKinlay M, Chunduru SK, Benetatos CA, Condon SM, Vince JE, Herold MJ, Silke J
Cell death and differentiation 2017 Mar;24(3):481-491
Cell death and differentiation 2017 Mar;24(3):481-491
Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice.
Guicciardi ME, Krishnan A, Bronk SF, Hirsova P, Griffith TS, Gores GJ
Cell death & disease 2017 Jan 5;8(1):e2535
Cell death & disease 2017 Jan 5;8(1):e2535
The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice.
Zheng J, Wu WL, Liu Y, Xiang Z, Liu M, Chan KH, Lau SY, Lam KT, To KK, Chan JF, Li L, Chen H, Lau YL, Yuen KY, Tu W
PloS one 2015;10(8):e0135999
PloS one 2015;10(8):e0135999
Reciprocal complementation of the tumoricidal effects of radiation and natural killer cells.
Yang KL, Wang YS, Chang CC, Huang SC, Huang YC, Chi MS, Chi KH
PloS one 2013;8(4):e61797
PloS one 2013;8(4):e61797
Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins.
Inoue T, Tashiro Y, Takeuchi M, Otani T, Tsuji-Takayama K, Okochi A, Mukae Y, Koreishi M, Yamasaki F, Kumon H, Nakamura S, Kibata M, Kondo E
International journal of oncology 2011 May;38(5):1299-306
International journal of oncology 2011 May;38(5):1299-306
KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
Navenot JM, Fujii N, Peiper SC
Molecular pharmacology 2009 May;75(5):1074-83
Molecular pharmacology 2009 May;75(5):1074-83
Inhibition of p38 mitogen-activated protein kinase unmasks a CD30-triggered apoptotic pathway in anaplastic large cell lymphoma cells.
Krysov SV, Rowley TF, Al-Shamkhani A
Molecular cancer therapeutics 2007 Feb;6(2):703-11
Molecular cancer therapeutics 2007 Feb;6(2):703-11
A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK
Cell death and differentiation 2006 Oct;13(10):1789-801
Cell death and differentiation 2006 Oct;13(10):1789-801
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Fas Ligand/TNFSF6 in HEK293 Human Cell Line Transfected with Human Fas Ligand/TNFSF6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) Fas Ligand/TNFSF6 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog # MAB126) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by Fas Ligand/TNFSF6 and Neutralization by Human Fas Ligand/TNFSF6 Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050), Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induces cytotoxicity in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Human Fas Ligand/TNFSF6 (2 ng/mL) is neutralized (green line) by increasing concentrations of Human Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog # MAB126). The ND50 is typically 1-5 ng/mL.
