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Immunocytochemistry
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-009-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-009-C100, RRID:AB_2051397
- Product name
- FasL (mouse) monoclonal antibody (A11)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgM
- Antibody clone number
- A11
- Vial size
- 100 μg
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Intracellular localization of keratinocyte Fas ligand explains lack of cytolytic activity under physiological conditions.
Fas ligand overexpression on allograft endothelium inhibits inflammatory cell infiltration and transplant-associated intimal hyperplasia.
The regulation of CD95 ligand expression and function in CTL.
Biochemical mechanisms of IL-2-regulated Fas-mediated T cell apoptosis.
Fas ligand expression by astrocytoma in vivo: maintaining immune privilege in the brain?
Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape.
Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand.
Fas and Fas ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover.
Characterization of the non-functional Fas ligand of gld mice.
Nitric oxide synthases in mammals.
Viard-Leveugle I, Bullani RR, Meda P, Micheau O, Limat A, Saurat JH, Tschopp J, French LE
The Journal of biological chemistry 2003 May 2;278(18):16183-8
The Journal of biological chemistry 2003 May 2;278(18):16183-8
Fas ligand overexpression on allograft endothelium inhibits inflammatory cell infiltration and transplant-associated intimal hyperplasia.
Sata M, Luo Z, Walsh K
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6964-71
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6964-71
The regulation of CD95 ligand expression and function in CTL.
Li JH, Rosen D, Ronen D, Behrens CK, Krammer PH, Clark WR, Berke G
Journal of immunology (Baltimore, Md. : 1950) 1998 Oct 15;161(8):3943-9
Journal of immunology (Baltimore, Md. : 1950) 1998 Oct 15;161(8):3943-9
Biochemical mechanisms of IL-2-regulated Fas-mediated T cell apoptosis.
Refaeli Y, Van Parijs L, London CA, Tschopp J, Abbas AK
Immunity 1998 May;8(5):615-23
Immunity 1998 May;8(5):615-23
Fas ligand expression by astrocytoma in vivo: maintaining immune privilege in the brain?
Saas P, Walker PR, Hahne M, Quiquerez AL, Schnuriger V, Perrin G, French L, Van Meir EG, de Tribolet N, Tschopp J, Dietrich PY
The Journal of clinical investigation 1997 Mar 15;99(6):1173-8
The Journal of clinical investigation 1997 Mar 15;99(6):1173-8
Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape.
Hahne M, Rimoldi D, Schröter M, Romero P, Schreier M, French LE, Schneider P, Bornand T, Fontana A, Lienard D, Cerottini J, Tschopp J
Science (New York, N.Y.) 1996 Nov 22;274(5291):1363-6
Science (New York, N.Y.) 1996 Nov 22;274(5291):1363-6
Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand.
Renno T, Hahne M, Tschopp J, MacDonald HR
The Journal of experimental medicine 1996 Feb 1;183(2):431-7
The Journal of experimental medicine 1996 Feb 1;183(2):431-7
Fas and Fas ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover.
French LE, Hahne M, Viard I, Radlgruber G, Zanone R, Becker K, Müller C, Tschopp J
The Journal of cell biology 1996 Apr;133(2):335-43
The Journal of cell biology 1996 Apr;133(2):335-43
Characterization of the non-functional Fas ligand of gld mice.
Hahne M, Peitsch MC, Irmler M, Schröter M, Lowin B, Rousseau M, Bron C, Renno T, French L, Tschopp J
International immunology 1995 Sep;7(9):1381-6
International immunology 1995 Sep;7(9):1381-6
Nitric oxide synthases in mammals.
Knowles RG, Moncada S
The Biochemical journal 1994 Mar 1;298 ( Pt 2):249-58
The Biochemical journal 1994 Mar 1;298 ( Pt 2):249-58
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- Experimental details
- Immunohistochemical localization of FasL in adult mouse testis using the A11 antibody. Method: Cryostat sections (6-8µm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 µg/ml of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope. Paraffin sections: Dewax in xylene (2x10 min), EtOH 100% (2x10 min), block endogeneous peroxidase (0.6% H2O2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H20, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2x5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 µg/ml antibody, wash in PBS, incubate in anti-rat IgM HRP (ALX-211-055), wash in PBS, develop in appropriate substrate solution (DAB or AEC).
