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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MAB5551 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB5551, RRID:AB_10680977
- Product name
- TRIM5 monoclonal antibody, clone 3B11H2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant TRIM5.
- Isotype
- IgG
- Antibody clone number
- 3B11H2
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Retrovirus restriction by TRIM5alpha variants from Old World and New World primates.
The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys.
Song B, Javanbakht H, Perron M, Park DH, Stremlau M, Sodroski J
Journal of virology 2005 Apr;79(7):3930-7
Journal of virology 2005 Apr;79(7):3930-7
The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys.
Stremlau M, Owens CM, Perron MJ, Kiessling M, Autissier P, Sodroski J
Nature 2004 Feb 26;427(6977):848-53
Nature 2004 Feb 26;427(6977):848-53
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using TRIM5 monoclonal antibody, clone 3B11H2 (Cat # MAB5551) against truncated TRIM5 recombinant protein.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using TRIM5 monoclonal antibody, clone 3B11H2 (Cat # MAB5551) against lysate of human breast tissue with Pierce's SuperSignal Femto substrate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded metastatic adenocarcinoma (A) showing cytoplasmic localization, human stomach adenocarcinoma (B) showing cytoplasmic localization, using TRIM5 monoclonal antibody, clone 3B11H2 (Cat # MAB5551).The second antibody HRP was used brfore color development with AEC (A) and DAB (B).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
