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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-1226 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1226, RRID:AB_10002120
- Product name
- Goat Polyclonal TRIM5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise all three human isoforms of this protein.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The tripartite motif family identifies cell compartments.
Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A
The EMBO journal 2001 May 1;20(9):2140-51
The EMBO journal 2001 May 1;20(9):2140-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIM5 Antibody [NB100-1226] - (2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226] - (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) positive cells next to a secondary follicle, B) positive cells below tonsillar epithelium.
