PA5-29316

antibody from Invitrogen Antibodies

Targeting: DDR1 CAK, CD167, EDDR1, NEP, NTRK4, PTK3A, RTK6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29316

Provider

Invitrogen Antibodies

Product name

MCK10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, H1299, HeLa, HepG2, Raji, mouse brain.

Concentration

1 mg/mL

References

Submitted references

Discoidin Domain Receptor 1 Regulates Runx2 during Osteogenesis of Osteoblasts and Promotes Bone Ossification via Phosphorylation of p38.

Chou LY, Chen CH, Chuang SC, Cheng TL, Lin YH, Chou HC, Fu YC, Wang YH, Wang CZ
International journal of molecular sciences 2020 Sep 29;21(19)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MCK10 Polyclonal Antibody (Product # PA5-29316). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 7.5% SDS PAGE. MCK10 Polyclonal Antibody (Product # PA5-29316) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MCK10 Polyclonal Antibody (Product # PA5-29316). Sample (30 µg of whole cell lysate). Lane A: H1299. 7.5% SDS PAGE. MCK10 Polyclonal Antibody (Product # PA5-29316) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DDR1 (Product # PA5-29316) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Generation of osteoblast-specific (a1(I) collagen-CreERT; Ddr1 f/f ) OKODelta Ddr1 mice. The calvarial bone was extracted from Ddr1 f/f-4OHT and OKODelta Ddr1 mice on postnatal days 4~5, and ( A ) gene expression of Ddr1 and ( B ) protein level of DDR1 were detected. The ratio of DDR1/GPADH is expressed relative to the Ddr1 f/f-4OHT mice was quantified. Each group n >= 6. ( C ) IHC staining of DDR1 and DDR2 in cortical bone and articular cartilage of femurs from 4-week-old mice; black arrow indicates DDR1-positive cells; black frame indicates the cortical region. ( D ) Quantitative results by tissue faxes normalized with Ddr1 f/f-4OHT . Magnifications of 400X are shown, with scale bars of 50 mum. Each group, n >= 6; * p