A700-084

antibody from Invitrogen Antibodies

Targeting: MED12 ARC240, CAGH45, FGS1, HOPA, KIAA0192, Kto, OKS, OPA1, TNRC11, TRAP230

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-084

Provider

Invitrogen Antibodies

Product name

MED12 Recombinant Rabbit Monoclonal Antibody (BLR084G)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR084G

Vial size

100 µL

Concentration

250 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse MED12 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, A-549, MCF-7, Hep-G2, K-562, RKO, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody [BLR084G] (Product # A700-084 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-eEF2 (Product # A301-688A).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MED12 in FFPE HCT-116 cells by ICC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody [BLR084G] (Product # A700-084; lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MED12 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (BLR084G) (A700-084 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse MED12 in FFPE renal carcinoma by IHC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody [BLR084G] (Product # A700-084 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MED12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED12 recombinant monoclonal antibody [BLR084G] (Product # A700-084 lot 1) used for IP at 20 µL/mg lysate. MED12 was also immunoprecipitated by rabbit anti-MED12 antibody (Product # A300-774A). For blotting immunoprecipitated MED12 (Product # A700-084) was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.