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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-681 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-681, RRID:AB_2233620
- Product name
- Anti-IL3RA
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Glycerol monolaurate prevents mucosal SIV transmission.
Induction of neutrophil gelatinase-associated lipocalin in vascular injury via activation of nuclear factor-kappaB.
IL-3 receptor signaling is dispensable for BCR-ABL-induced myeloproliferative disease.
Li Q, Estes JD, Schlievert PM, Duan L, Brosnahan AJ, Southern PJ, Reilly CS, Peterson ML, Schultz-Darken N, Brunner KG, Nephew KR, Pambuccian S, Lifson JD, Carlis JV, Haase AT
Nature 2009 Apr 23;458(7241):1034-8
Nature 2009 Apr 23;458(7241):1034-8
Induction of neutrophil gelatinase-associated lipocalin in vascular injury via activation of nuclear factor-kappaB.
Bu DX, Hemdahl AL, Gabrielsen A, Fuxe J, Zhu C, Eriksson P, Yan ZQ
The American journal of pathology 2006 Dec;169(6):2245-53
The American journal of pathology 2006 Dec;169(6):2245-53
IL-3 receptor signaling is dispensable for BCR-ABL-induced myeloproliferative disease.
Wong S, McLaughlin J, Cheng D, Shannon K, Robb L, Witte ON
Proceedings of the National Academy of Sciences of the United States of America 2003 Sep 30;100(20):11630-5
Proceedings of the National Academy of Sciences of the United States of America 2003 Sep 30;100(20):11630-5
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 19
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human hippocampus shows cytoplasmic and nucleolar positivity in neuronal cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
