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Learnsc-17774
antibody from Santa Cruz Biotechnology
Targeting: APEX1
APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-17774 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-17774, RRID:AB_626685
- Product name
- Anti-APEX1
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Immunohistochemical analysis of oxidative stress and DNA repair proteins in normal mammary and breast cancer tissues.
Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2.
Apurinic/apyrimidinic endonuclease 1 alters estrogen receptor activity and estrogen-responsive gene expression.
Immunohistochemical profiling of benign, low malignant potential and low grade serous epithelial ovarian tumors.
ATF4-dependent oxidative induction of the DNA repair enzyme Ape1 counteracts arsenite cytotoxicity and suppresses arsenite-mediated mutagenesis.
Curtis CD, Thorngren DL, Nardulli AM
BMC cancer 2010 Jan 11;10:9
BMC cancer 2010 Jan 11;10:9
Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2.
Ku WC, Chiu SK, Chen YJ, Huang HH, Wu WG, Chen YJ
Molecular & cellular proteomics : MCP 2009 Sep;8(9):2034-50
Molecular & cellular proteomics : MCP 2009 Sep;8(9):2034-50
Apurinic/apyrimidinic endonuclease 1 alters estrogen receptor activity and estrogen-responsive gene expression.
Curtis CD, Thorngren DL, Ziegler YS, Sarkeshik A, Yates JR, Nardulli AM
Molecular endocrinology (Baltimore, Md.) 2009 Sep;23(9):1346-59
Molecular endocrinology (Baltimore, Md.) 2009 Sep;23(9):1346-59
Immunohistochemical profiling of benign, low malignant potential and low grade serous epithelial ovarian tumors.
Ouellet V, Ling TH, Normandin K, Madore J, Lussier C, Barrès V, Bachvarov D, Rancourt C, Tonin PN, Provencher DM, Mes-Masson AM
BMC cancer 2008 Nov 26;8:346
BMC cancer 2008 Nov 26;8:346
ATF4-dependent oxidative induction of the DNA repair enzyme Ape1 counteracts arsenite cytotoxicity and suppresses arsenite-mediated mutagenesis.
Fung H, Liu P, Demple B
Molecular and cellular biology 2007 Dec;27(24):8834-47
Molecular and cellular biology 2007 Dec;27(24):8834-47
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 36
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
