- GTX110558 - GeneTex APEX1 antibody

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
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Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 40044

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: APE1 antibody detects APEX1 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract10% SDS-PAGEAPE1 antibody (GTX110558) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: Hela C: Molt-4 (GTX27912) 10% SDS PAGE GTX110558 diluted at 1:1000

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE GTX110558 diluted at 1:2000

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: Mouse tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: Immunofluorescence analysis of paraformaldehyde-fixed A549, using APE1(GTX110558) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: APE1 antibody immunoprecipitates APE1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : 30 £gg whole cell lysate/extract of APE1 protein expressing A431 cells B : Control with 3 £gg of pre-immune rabbit IgG C : Immunoprecipitation of APE1 by 3 £gg of APE1 antibody (GTX110558) 10% SDS-PAGE The immunoprecipitated APE1 protein was detected by APE1 antibody (GTX110558) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: APE1 antibody detects APE1 protein at cytoplasm, nucleus and nucleolus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. APE1 antibody (GTX110558) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
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Experimental details: Cross-linked ChIP was performed with 293T chromatin extract treated with Trichostatin A (0.4 £gM for 18 h) and 5 £gg of either control rabbit IgG or anti-APE1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MDR1 promoter.

GTX110558