AP2849a

antibody from Abgent

Targeting: APEX1 APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2849a

Provider

Abgent

Proper citation

Abgent Cat#AP2849a, RRID:AB_1967233

Product name

APEX1 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-APEX1 Antibody (N-term) (Cat.#AP2849a) in Hela cell line lysates (35ug/lane). APEX1 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of APEX1 (arrow) using rabbit polyclonal APEX1 Antibody (N-term) (Cat.#AP2849a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APEX1 gene.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of APEX1 Antibody (N-term)(Cat#AP2849a) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50