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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP7707a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP7707a, RRID:AB_2103956
- Product name
- FGR Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references High-throughput RNAi screening identifies a role for TNK1 in growth and survival of pancreatic cancer cells.
SRC family kinase activity is up-regulated in hormone-refractory prostate cancer.
Henderson MC, Gonzales IM, Arora S, Choudhary A, Trent JM, Von Hoff DD, Mousses S, Azorsa DO
Molecular cancer research : MCR 2011 Jun;9(6):724-32
Molecular cancer research : MCR 2011 Jun;9(6):724-32
SRC family kinase activity is up-regulated in hormone-refractory prostate cancer.
Tatarov O, Mitchell TJ, Seywright M, Leung HY, Brunton VG, Edwards J
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 May 15;15(10):3540-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 May 15;15(10):3540-9
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- The anti-FGR Pab (Cat. #AP7707a) is used in Western blot to detect FGR in Ramos cell lysate.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of FGR (arrow) using FGR Antibody (N-term) (Cat.#AP7707a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGR gene (Lane 2) (Origene Technologies).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of FGR Antibody (N-term)(Cat#AP7707a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
