NBP1-54578

antibody from Novus Biologicals

Targeting: TXNIP ARRDC6, EST01027, HHCPA78, THIF, VDUP1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

NBP1-54578

Provider

Novus Biologicals

Product name

Mouse Monoclonal TXNIP Antibody

Antibody type

Monoclonal

Description

Protein A or G purified.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

8-Chloroadenosine Alters the Metabolic Profile and Downregulates Antioxidant and DNA Damage Repair Pathways in Macrophages.

Macer-Wright JL, Sileikaite I, Rayner BS, Hawkins CL
Chemical research in toxicology 2020 Feb 17;33(2):402-413

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Silica dioxide nanoparticles aggravate airway inflammation in an asthmatic mouse model via NLRP3 inflammasome activation.

Ko JW, Shin NR, Je-Oh L, Jung TY, Moon C, Kim TW, Choi J, Shin IS, Heo JD, Kim JC
Regulatory toxicology and pharmacology : RTP 2020 Apr;112:104618

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Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway.

Wei H, Bu R, Yang Q, Jia J, Li T, Wang Q, Chen Y
Journal of diabetes research 2019;2019:8905917

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Fenofibrate Rescues Diabetes-Related Impairment of Ischemia-Mediated Angiogenesis by PPARα-Independent Modulation of Thioredoxin-Interacting Protein.

Yuan J, Tan JTM, Rajamani K, Solly EL, King EJ, Lecce L, Simpson PJL, Lam YT, Jenkins AJ, Bursill CA, Keech AC, Ng MKC
Diabetes 2019 May;68(5):1040-1053

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Reactive Oxygen Species Evoked by Potassium Deprivation and Staurosporine Inactivate Akt and Induce the Expression of TXNIP in Cerebellar Granule Neurons.

Zaragoza-Campillo MA, Morán J
Oxidative medicine and cellular longevity 2017;2017:8930406

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Ginsenoside Rb1 and compound K improve insulin signaling and inhibit ER stress-associated NLRP3 inflammasome activation in adipose tissue.

Chen W, Wang J, Luo Y, Wang T, Li X, Li A, Li J, Liu K, Liu B
Journal of ginseng research 2016 Oct;40(4):351-358

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Response of esophageal cancer cells to epigenetic inhibitors is mediated via altered thioredoxin activity.

Ahrens TD, Timme S, Ostendorp J, Bogatyreva L, Hoeppner J, Hopt UT, Hauschke D, Werner M, Lassmann S
Laboratory investigation; a journal of technical methods and pathology 2016 Mar;96(3):307-16

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Ilexgenin A inhibits endoplasmic reticulum stress and ameliorates endothelial dysfunction via suppression of TXNIP/NLRP3 inflammasome activation in an AMPK dependent manner.

Li Y, Yang J, Chen MH, Wang Q, Qin MJ, Zhang T, Chen XQ, Liu BL, Wen XD
Pharmacological research 2015 Sep;99:101-15

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Endoplasmic Reticulum Stress Activates the Inflammasome via NLRP3- and Caspase-2-Driven Mitochondrial Damage.

Bronner DN, Abuaita BH, Chen X, Fitzgerald KA, Nuñez G, He Y, Yin XM, O'Riordan MX
Immunity 2015 Sep 15;43(3):451-62

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Activation of the NLRP3 inflammasome in association with calcium oxalate crystal induced reactive oxygen species in kidneys.

Joshi S, Wang W, Peck AB, Khan SR
The Journal of urology 2015 May;193(5):1684-91

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Mangiferin inhibits endoplasmic reticulum stress-associated thioredoxin-interacting protein/NLRP3 inflammasome activation with regulation of AMPK in endothelial cells.

Song J, Li J, Hou F, Wang X, Liu B
Metabolism: clinical and experimental 2015 Mar;64(3):428-37

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Umbelliferone ameliorates cerebral ischemia-reperfusion injury via upregulating the PPAR gamma expression and suppressing TXNIP/NLRP3 inflammasome.

Wang X, Li R, Wang X, Fu Q, Ma S
Neuroscience letters 2015 Jul 23;600:182-7

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Curcumin attenuates glutamate neurotoxicity in the hippocampus by suppression of ER stress-associated TXNIP/NLRP3 inflammasome activation in a manner dependent on AMPK.

Li Y, Li J, Li S, Li Y, Wang X, Liu B, Fu Q, Ma S
Toxicology and applied pharmacology 2015 Jul 1;286(1):53-63

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Astragaloside IV and cycloastragenol are equally effective in inhibition of endoplasmic reticulum stress-associated TXNIP/NLRP3 inflammasome activation in the endothelium.

Zhao Y, Li Q, Zhao W, Li J, Sun Y, Liu K, Liu B, Zhang N
Journal of ethnopharmacology 2015 Jul 1;169:210-8

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Quercetin, luteolin and epigallocatechin gallate alleviate TXNIP and NLRP3-mediated inflammation and apoptosis with regulation of AMPK in endothelial cells.

Wu J, Xu X, Li Y, Kou J, Huang F, Liu B, Liu K
European journal of pharmacology 2014 Dec 15;745:59-68

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Intracellular shuttling and mitochondrial function of thioredoxin-interacting protein.

Saxena G, Chen J, Shalev A
The Journal of biological chemistry 2010 Feb 5;285(6):3997-4005

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Diabetes induces and calcium channel blockers prevent cardiac expression of proapoptotic thioredoxin-interacting protein.

Chen J, Cha-Molstad H, Szabo A, Shalev A
American journal of physiology. Endocrinology and metabolism 2009 May;296(5):E1133-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP expression in Raji (1), K562 (2), KG1 (3), MRC5 (4), IC2Tr (5), HEL (6), P19 (7) and WR19L (8).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - Hepa1 lysates probed with anti-TXNIP (JY2) at 1:2K. Cells were transfected with either empty vector or mouse TXNIP expression vector (MYC/DDK tagged). 30ug protein per lane. Image from verified customer review.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: TXNIP Antibody (JY2) [NBP1-54578] - Txnip/VDUP1 from Raji with NBP1-54578 (1) or mouse IgG1 (2).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:100 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counterstaining of the cell nuclei with hematoxylin. This TXNIP antibody generated a diffused cytoplasmic signal in the cells of various tubules with negligible staining in glomeruli.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:250 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counterstaining of the cell nuclei with hematoxylin. This TXNIP antibody generated a diffused cytoplasmic signal in the cells of various tubules and glomeruli.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP in mouse brain. Antibody dilution: 1:100, incubated overnight in 5%BSA + 0.2% Triton X 100. Image from verified customer review.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: TXNIP Antibody (JY2) [NBP1-54578] - An intracellular stain was performed on RH-30 cells with TXNIP (JY2) Antibody NBP1-54578F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.