NBP2-33172

antibody from Novus Biologicals

Targeting: PMEL D12S53E, gp100, Pmel17, SI, SIL, SILV

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Data presented on provider website)

Antibody Data

Product number

NBP2-33172

Provider

Novus Biologicals

Product name

Mouse Monoclonal PMEL17/SILV Antibody

Antibody type

Monoclonal

Description

Protein A purified. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172] - Formalin-paraffin human melanoma tissue stained with gp100 Ab (NKI-beteb)

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172] - Formalin-fixed, paraffin-embedded human melanoma tissue stained with gp100 NKI-beteb antibody, peroxidase-conjugate and DAB chromogen.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172] - An intracellular stain was performed on SK-MEL-28 cells with PMEL/SILV [NKI-beteb] Antibody NBP2-33172AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 format of this antibody.