Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- OAAB17264 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- B - RAF antibody - center region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of BRAF Antibody (Center) polyclonal antibody(Cat.# OAAB17264) (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies). BRAF is strongly supported by BioGPS gene expression data to be expressed in HEK293
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human prostata carcinoma reacted with BRAF Antibody (Center) (Cat.# OAAB17264) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- B-RAF Antibody (Center) (Cat. # OAAB17264) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram) .FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.