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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
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- Product number
- 14-9791-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-TRAF-1 Monoclonal Antibody (1F3), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 1F3 recognizes human TRAF-1. TRAF-1 is a multifunctional adapter molecule and member of the TNF receptor associated factor protein family. TRAF proteins associate with various receptors of the TNF receptor superfamily to mediate signal transduction. TRAF-1 plays a role in regulating cell survival and apoptosis, regulates the signaling of NF kappa B and JNK, and forms a heterodimeric complex with TRAF-2. TRAF-1 also interacts with cell surface receptors such as CD30, CD40, and CD357 (AITR), and cytoplasmic proteins like NIK, RIP2, A20, and TRIP. Expression of TRAF-1 can be induced by Epstein-Barr Virus (EBV) and TNF alpha. Applications Reported: This 1F3 antibody has been reported for use in western blotting, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This 1F3 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 1F3
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Expression of tumor necrosis factor receptor-associated factor 1 in nasopharyngeal carcinoma: possible upregulation by Epstein-Barr virus latent membrane protein 1.
Epstein-Barr virus encoded latent membrane protein 1 (LMP1) and TNF receptor associated factors (TRAF): colocalisation of LMP1 and TRAF1 in primary EBV infection and in EBV associated Hodgkin lymphoma.
A regulatory role for TRAF1 in antigen-induced apoptosis of T cells.
Siegler G, Meyer B, Dawson C, Brachtel E, Lennerz J, Koch C, Kremmer E, Niedobitek E, Gonnella R, Pilch BZ, Young LS, Niedobitek G
International journal of cancer 2004 Nov 1;112(2):265-72
International journal of cancer 2004 Nov 1;112(2):265-72
Epstein-Barr virus encoded latent membrane protein 1 (LMP1) and TNF receptor associated factors (TRAF): colocalisation of LMP1 and TRAF1 in primary EBV infection and in EBV associated Hodgkin lymphoma.
Siegler G, Kremmer E, Gonnella R, Niedobitek G
Molecular pathology : MP 2003 Jun;56(3):156-61
Molecular pathology : MP 2003 Jun;56(3):156-61
A regulatory role for TRAF1 in antigen-induced apoptosis of T cells.
Speiser DE, Lee SY, Wong B, Arron J, Santana A, Kong YY, Ohashi PS, Choi Y
The Journal of experimental medicine 1997 May 19;185(10):1777-83
The Journal of experimental medicine 1997 May 19;185(10):1777-83
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- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 5 µg/mL of Rat IgG2a K Isotype Control Purified (left) or 5 µg/mL of Anti-Human TRAF-1 Purified (right) followed by Anti-Rat Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
