26845-1-AP

antibody from Invitrogen Antibodies

Targeting: TRAF1 EBI6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

26845-1-AP

Provider

Invitrogen Antibodies

Product name

TRAF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: QEELALQHF MKEKLLAELE GKLRVFENIV AVLNKEVEAS HLALATSIHQ SQLDRERILS LEQRVVELQQ TLAQKDQALG KLEQSLRLME EASFDGTFLW KITNVTRRCH ESACGRTVSL FSPA (173-295 aa encoded by B C024145)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 26845-1-AP ( TRAF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HUVEC cells using 26845-1-AP (TRAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HUVEC cells using 26845-1-AP (TRAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen tissue slide using 26845-1-AP (TRAF1 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen tissue slide using 26845-1-AP (TRAF1 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 26845-1-AP ( TRAF1 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 26845-1-AP ( TRAF1 Antibody) at dilution of 1:200 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 Raji cells were intracellularly stained with 0.20 µg/test Anti-Human TRAF1 (Product # 26845-1-AP) (red) or 0.20 µg control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1,000. Fixed with 90% MeOH.