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LearnCF506840
antibody from Invitrogen Antibodies
Targeting: CHAF1B
CAF-1, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- CF506840 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHAF1B Monoclonal Antibody (OTI4F7), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI4F7
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAF1B (RC202858, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CHAF1. Positive lysates LY401669 (100 µg) and LC401669 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-CHAF1B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-CHAF1B mouse monoclonal antibody (TA506840) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B(RC202858).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-CHAF1B mouse monoclonal antibody (TA506840) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B(RC202858).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506840)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lymph node tissue within the normal limits using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506840)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506840)
