A00878-1

antibody from Boster Biological Technology

Targeting: DYRK1A DYRK, DYRK1, MNBH

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00878-1

Provider

Boster Biological Technology

Product name

Anti-DYRK1A Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for DYRK1A detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, ICC/IF, FCM, Direct ELISA. Reactive species: Human. DYRK1A information: Subcellular Localization: Nucleus; Tissue Specificity: Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of SiHa cells using anti- DYRK1A antibody (A00878-1). Overlay histogram showing SiHa cells stained with A00878-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DYRK1A Antibody (A00878-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image