H00001859-M01

antibody from Novus Biologicals

Targeting: DYRK1A DYRK, DYRK1, MNBH

Provider product page for H00001859-M01

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Functional assay

Antibody data

Antibody Data
Antigen structure
References [53]
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Validations
Western blot [4]
ELISA [1]

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Product number: H00001859-M01 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#H00001859-M01, RRID:AB_2230676
Product name: Mouse Monoclonal DYRK1A Antibody
Antibody type: Monoclonal
Description: IgG purified. DYRK1A - dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Vial size: 0.1 mg
Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

DYRK1A protein structure - H00001859-M01 shown in red.

Submitted references Inhibition of DYRK1A proteolysis modifies its kinase specificity and rescues Alzheimer phenotype in APP/PS1 mice.

Souchet B, Audrain M, Billard JM, Dairou J, Fol R, Orefice NS, Tada S, Gu Y, Dufayet-Chaffaud G, Limanton E, Carreaux F, Bazureau JP, Alves S, Meijer L, Janel N, Braudeau J, Cartier N
Acta neuropathologica communications 2019 Mar 18;7(1):46

LPS-Induced Inflammation Abolishes the Effect of DYRK1A on IkB Stability in the Brain of Mice.

Latour A, Gu Y, Kassis N, Daubigney F, Colin C, Gausserès B, Middendorp S, Paul JL, Hindié V, Rain JC, Delabar JM, Yu E, Arbones M, Mallat M, Janel N
Molecular neurobiology 2019 Feb;56(2):963-975

A comprehensive proteomics-based interaction screen that links DYRK1A to RNF169 and to the DNA damage response.

Roewenstrunk J, Di Vona C, Chen J, Borras E, Dong C, Arató K, Sabidó E, Huen MSY, de la Luna S
Scientific reports 2019 Apr 12;9(1):6014

Homocysteine-lowering gene therapy rescues signaling pathways in brain of mice with intermediate hyperhomocysteinemia.

Baloula V, Fructuoso M, Kassis N, Gueddouri D, Paul JL, Janel N
Redox biology 2018 Oct;19:200-209

Splicing Factor 3B Subunit 1 Interacts with HIV Tat and Plays a Role in Viral Transcription and Reactivation from Latency.

Kyei GB, Meng S, Ramani R, Niu A, Lagisetti C, Webb TR, Ratner L
mBio 2018 Nov 6;9(6)

Overexpression of the DYRK1A Gene (Dual-Specificity Tyrosine Phosphorylation-Regulated Kinase 1A) Induces Alterations of the Serotoninergic and Dopaminergic Processing in Murine Brain Tissues.

London J, Rouch C, Bui LC, Assayag E, Souchet B, Daubigney F, Medjaoui H, Luquet S, Magnan C, Delabar JM, Dairou J, Janel N
Molecular neurobiology 2018 May;55(5):3822-3831

Cerebellar alterations in a model of Down syndrome: The role of the Dyrk1A gene.

García-Cerro S, Vidal V, Lantigua S, Berciano MT, Lafarga M, Ramos-Cabrer P, Padro D, Rueda N, Martínez-Cué C
Neurobiology of disease 2018 Feb;110:206-217

Autism-like phenotype and risk gene mRNA deadenylation by CPEB4 mis-splicing.

Parras A, Anta H, Santos-Galindo M, Swarup V, Elorza A, Nieto-González JL, Picó S, Hernández IH, Díaz-Hernández JI, Belloc E, Rodolosse A, Parikshak NN, Peñagarikano O, Fernández-Chacón R, Irimia M, Navarro P, Geschwind DH, Méndez R, Lucas JJ
Nature 2018 Aug;560(7719):441-446

Combined assessment of DYRK1A, BDNF and homocysteine levels as diagnostic marker for Alzheimer's disease.

Janel N, Alexopoulos P, Badel A, Lamari F, Camproux AC, Lagarde J, Simon S, Feraudet-Tarisse C, Lamourette P, Arbones M, Paul JL, Dubois B, Potier MC, Sarazin M, Delabar JM
Translational psychiatry 2017 Jun 20;7(6):e1154

The adaptor protein DCAF7 mediates the interaction of the adenovirus E1A oncoprotein with the protein kinases DYRK1A and HIPK2.

Glenewinkel F, Cohen MJ, King CR, Kaspar S, Bamberg-Lemper S, Mymryk JS, Becker W
Scientific reports 2016 Jun 16;6:28241

Altered regulation of the Spry2/Dyrk1A/PP2A triad by homocysteine impairs neural progenitor cell proliferation.

Rabaneda LG, Geribaldi-Doldán N, Murillo-Carretero M, Carrasco M, Martínez-Salas JM, Verástegui C, Castro C
Biochimica et biophysica acta 2016 Dec;1863(12):3015-3026

DYRK1A-mediated Cyclin D1 Degradation in Neural Stem Cells Contributes to the Neurogenic Cortical Defects in Down Syndrome.

Najas S, Arranz J, Lochhead PA, Ashford AL, Oxley D, Delabar JM, Cook SJ, Barallobre MJ, Arbonés ML
EBioMedicine 2015;2(2):120-34

DYRK1A Controls HIV-1 Replication at a Transcriptional Level in an NFAT Dependent Manner.

Booiman T, Loukachov VV, van Dort KA, van 't Wout AB, Kootstra NA
PloS one 2015;10(12):e0144229

Rescue of the abnormal skeletal phenotype in Ts65Dn Down syndrome mice using genetic and therapeutic modulation of trisomic Dyrk1a.

Blazek JD, Abeysekera I, Li J, Roper RJ
Human molecular genetics 2015 Oct 15;24(20):5687-96

DYRK1A overexpression enhances STAT activity and astrogliogenesis in a Down syndrome mouse model.

Kurabayashi N, Nguyen MD, Sanada K
EMBO reports 2015 Nov;16(11):1548-62

Low dose EGCG treatment beginning in adolescence does not improve cognitive impairment in a Down syndrome mouse model.

Stringer M, Abeysekera I, Dria KJ, Roper RJ, Goodlett CR
Pharmacology, biochemistry, and behavior 2015 Nov;138:70-9

Corrective effects of hepatotoxicity by hepatic Dyrk1a gene delivery in mice with intermediate hyperhomocysteinemia.

Latour A, Salameh S, Carbonne C, Daubigney F, Paul JL, Kergoat M, Autier V, Delabar JM, De Geest B, Janel N
Molecular genetics and metabolism reports 2015 Mar;2:51-60

DYRK1A controls the transition from proliferation to quiescence during lymphoid development by destabilizing Cyclin D3.

Thompson BJ, Bhansali R, Diebold L, Cook DE, Stolzenburg L, Casagrande AS, Besson T, Leblond B, Désiré L, Malinge S, Crispino JD
The Journal of experimental medicine 2015 Jun 1;212(6):953-70

Molecular rescue of DYRK1A overexpression in cystathionine beta synthase-deficient mouse brain by enriched environment combined with voluntary exercise.

Souchet B, Latour A, Gu Y, Daubigney F, Paul JL, Delabar JM, Janel N
Journal of molecular neuroscience : MN 2015 Feb;55(2):318-23

Overexpression of Dyrk1A is implicated in several cognitive, electrophysiological and neuromorphological alterations found in a mouse model of Down syndrome.

García-Cerro S, Martínez P, Vidal V, Corrales A, Flórez J, Vidal R, Rueda N, Arbonés ML, Martínez-Cué C
PloS one 2014;9(9):e106572

DYRK1A-mediated phosphorylation of GluN2A at Ser(1048) regulates the surface expression and channel activity of GluN1/GluN2A receptors.

Grau C, Arató K, Fernández-Fernández JM, Valderrama A, Sindreu C, Fillat C, Ferrer I, de la Luna S, Altafaj X
Frontiers in cellular neuroscience 2014;8:331

One-carbon cycle alterations induced by Dyrk1a dosage.

Delabar JM, Latour A, Noll C, Renon M, Salameh S, Paul JL, Arbones M, Movassat J, Janel N
Molecular genetics and metabolism reports 2014;1:487-492

Dyrk1a haploinsufficiency induces diabetes in mice through decreased pancreatic beta cell mass.

Rachdi L, Kariyawasam D, Guez F, Aïello V, Arbonés ML, Janel N, Delabar JM, Polak M, Scharfmann R
Diabetologia 2014 May;57(5):960-9

cdc-like/dual-specificity tyrosine phosphorylation-regulated kinases inhibitor leucettine L41 induces mTOR-dependent autophagy: implication for Alzheimer's disease.

Fant X, Durieu E, Chicanne G, Payrastre B, Sbrissa D, Shisheva A, Limanton E, Carreaux F, Bazureau JP, Meijer L
Molecular pharmacology 2014 Mar;85(3):441-50

Prefrontal deficits in a murine model overexpressing the down syndrome candidate gene dyrk1a.

Thomazeau A, Lassalle O, Iafrati J, Souchet B, Guedj F, Janel N, Chavis P, Delabar J, Manzoni OJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jan 22;34(4):1138-47

Modelling and rescuing neurodevelopmental defect of Down syndrome using induced pluripotent stem cells from monozygotic twins discordant for trisomy 21.

Hibaoui Y, Grad I, Letourneau A, Sailani MR, Dahoun S, Santoni FA, Gimelli S, Guipponi M, Pelte MF, Béna F, Antonarakis SE, Feki A
EMBO molecular medicine 2014 Feb;6(2):259-77

Plasma DYRK1A as a novel risk factor for Alzheimer's disease.

Janel N, Sarazin M, Corlier F, Corne H, de Souza LC, Hamelin L, Aka A, Lagarde J, Blehaut H, Hindié V, Rain JC, Arbones ML, Dubois B, Potier MC, Bottlaender M, Delabar JM
Translational psychiatry 2014 Aug 12;4(8):e425

NGF upregulates the plasminogen activation inhibitor-1 in neurons via the calcineurin/NFAT pathway and the Down syndrome-related proteins DYRK1A and RCAN1 attenuate this effect.

Stefos GC, Soppa U, Dierssen M, Becker W
PloS one 2013;8(6):e67470

Dyrk1A is dynamically expressed on subsets of motor neurons and in the neuromuscular junction: possible role in Down syndrome.

Arque G, Casanovas A, Dierssen M
PloS one 2013;8(1):e54285

Mice deficient in cystathionine beta synthase display increased Dyrk1A and SAHH activities in brain.

Planque C, Dairou J, Noll C, Bui LC, Ripoll C, Guedj F, Delabar JM, Janel N
Journal of molecular neuroscience : MN 2013 May;50(1):1-6

Hepatocyte-specific Dyrk1a gene transfer rescues plasma apolipoprotein A-I levels and aortic Akt/GSK3 pathways in hyperhomocysteinemic mice.

Tlili A, Jacobs F, de Koning L, Mohamed S, Bui LC, Dairou J, Belin N, Ducros V, Dubois T, Paul JL, Delabar JM, De Geest B, Janel N
Biochimica et biophysica acta 2013 Jun;1832(6):718-28

Triplication of DYRK1A causes retinal structural and functional alterations in Down syndrome.

Laguna A, Barallobre MJ, Marchena MÁ, Mateus C, Ramírez E, Martínez-Cue C, Delabar JM, Castelo-Branco M, de la Villa P, Arbonés ML
Human molecular genetics 2013 Jul 15;22(14):2775-84

Expression of trisomic proteins in Down syndrome model systems.

Spellman C, Ahmed MM, Dubach D, Gardiner KJ
Gene 2013 Jan 10;512(2):219-25

Dyrk1A, a serine/threonine kinase, is involved in ERK and Akt activation in the brain of hyperhomocysteinemic mice.

Abekhoukh S, Planque C, Ripoll C, Urbaniak P, Paul JL, Delabar JM, Janel N
Molecular neurobiology 2013 Feb;47(1):105-16

Environmental enrichment rescues DYRK1A activity and hippocampal adult neurogenesis in TgDyrk1A.

Pons-Espinal M, Martinez de Lagran M, Dierssen M
Neurobiology of disease 2013 Dec;60:18-31

Normalization of Dyrk1A expression by AAV2/1-shDyrk1A attenuates hippocampal-dependent defects in the Ts65Dn mouse model of Down syndrome.

Altafaj X, Martín ED, Ortiz-Abalia J, Valderrama A, Lao-Peregrín C, Dierssen M, Fillat C
Neurobiology of disease 2013 Apr;52:117-27

The NAD(+)-dependent protein deacetylase activity of SIRT1 is regulated by its oligomeric status.

Guo X, Kesimer M, Tolun G, Zheng X, Xu Q, Lu J, Sheehan JK, Griffith JD, Li X
Scientific reports 2012;2:640

Increased dosage of the chromosome 21 ortholog Dyrk1a promotes megakaryoblastic leukemia in a murine model of Down syndrome.

Malinge S, Bliss-Moreau M, Kirsammer G, Diebold L, Chlon T, Gurbuxani S, Crispino JD
The Journal of clinical investigation 2012 Mar;122(3):948-62

Dyrk1A negatively regulates the actin cytoskeleton through threonine phosphorylation of N-WASP.

Park J, Sung JY, Park J, Song WJ, Chang S, Chung KC
Journal of cell science 2012 Jan 1;125(Pt 1):67-80

Loss of correlations among proteins in brains of the Ts65Dn mouse model of down syndrome.

Ahmed MM, Sturgeon X, Ellison M, Davisson MT, Gardiner KJ
Journal of proteome research 2012 Feb 3;11(2):1251-63

DYRK1A: a master regulatory protein controlling brain growth.

Guedj F, Pereira PL, Najas S, Barallobre MJ, Chabert C, Souchet B, Sebrie C, Verney C, Herault Y, Arbones M, Delabar JM
Neurobiology of disease 2012 Apr;46(1):190-203

Altered regulation of tau phosphorylation in a mouse model of down syndrome aging.

Sheppard O, Plattner F, Rubin A, Slender A, Linehan JM, Brandner S, Tybulewicz VL, Fisher EM, Wiseman FK
Neurobiology of aging 2012 Apr;33(4):828.e31-44

Form of dual-specificity tyrosine-(Y)-phosphorylation-regulated kinase 1A nonphosphorylated at tyrosine 145 and 147 is enriched in the nuclei of astroglial cells, adult hippocampal progenitors, and some cholinergic axon terminals.

Kida E, Walus M, Jarząbek K, Palminiello S, Albertini G, Rabe A, Hwang YW, Golabek AA
Neuroscience 2011 Nov 10;195:112-27

Engineering DYRK1A overdosage yields Down syndrome-characteristic cortical splicing aberrations.

Toiber D, Azkona G, Ben-Ari S, Torán N, Soreq H, Dierssen M
Neurobiology of disease 2010 Oct;40(1):348-59

Hyperhomocysteinemia-induced Dyrk1a downregulation results in cardiomyocyte hypertrophy in rats.

Raaf L, Noll C, Cherifi M, Benazzoug Y, Delabar JM, Janel N
International journal of cardiology 2010 Nov 19;145(2):306-307

DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1.

Guo X, Williams JG, Schug TT, Li X
The Journal of biological chemistry 2010 Apr 23;285(17):13223-32

Green tea polyphenols rescue of brain defects induced by overexpression of DYRK1A.

Guedj F, Sébrié C, Rivals I, Ledru A, Paly E, Bizot JC, Smith D, Rubin E, Gillet B, Arbones M, Delabar JM
PloS one 2009;4(2):e4606

DYRK1A, a novel determinant of the methionine-homocysteine cycle in different mouse models overexpressing this Down-syndrome-associated kinase.

Noll C, Planque C, Ripoll C, Guedj F, Diez A, Ducros V, Belin N, Duchon A, Paul JL, Badel A, de Freminville B, Grattau Y, Bléhaut H, Herault Y, Janel N, Delabar JM
PloS one 2009 Oct 21;4(10):e7540

Negative feedback Inhibition of NFATc1 by DYRK1A regulates bone homeostasis.

Lee Y, Ha J, Kim HJ, Kim YS, Chang EJ, Song WJ, Kim HH
The Journal of biological chemistry 2009 Nov 27;284(48):33343-51

Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons.

Scales TM, Lin S, Kraus M, Goold RG, Gordon-Weeks PR
Journal of cell science 2009 Jul 15;122(Pt 14):2424-35

Effect of hyperhomocysteinemia on the protein kinase DYRK1A in liver of mice.

Hamelet J, Noll C, Ripoll C, Paul JL, Janel N, Delabar JM
Biochemical and biophysical research communications 2009 Jan 16;378(3):673-7

Sprouty2-mediated inhibition of fibroblast growth factor signaling is modulated by the protein kinase DYRK1A.

Aranda S, Alvarez M, Turró S, Laguna A, de la Luna S
Molecular and cellular biology 2008 Oct;28(19):5899-911

High density lipoprotein metabolism in endurance athletes and sedentary men.

Thompson PD, Cullinane EM, Sady SP, Flynn MM, Chenevert CB, Herbert PN
Circulation 1991 Jul;84(1):140-52

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DYRK1A Antibody (7D10) [H00001859-M01] - DYRK1A monoclonal antibody (M01), clone 7D10 Western Blot analysis of DYRK1A expression in Hela NE ( Cat # L013V3 ).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DYRK1A Antibody (7D10) [H00001859-M01] - DYRK1A monoclonal antibody (M01), clone 7D10. Western Blot analysis of DYRK1A expression in 293 ( Cat # L026V1 ).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DYRK1A Antibody (7D10) [H00001859-M01] - DYRK1A monoclonal antibody (M01), clone 7D10. Western Blot analysis of DYRK1A expression in NIH/3T3 ( Cat # L018V1 ).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DYRK1A Antibody (7D10) [H00001859-M01] - DYRK1A monoclonal antibody (M01), clone 7D10. Analysis of DYRK1A expression in Hela S3 NE.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: ELISA: DYRK1A Antibody (7D10) [H00001859-M01] - Detection limit for recombinant GST tagged DYRK1A is approximately 0.03ng/ml as a capture antibody.