V3499

antibody from NSJ Bioreagents

Targeting: F13A1 F13A

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3499

Provider

NSJ Bioreagents

Product name

Factor XIIIa Antibody / F13A1

Antibody type

Monoclonal

Description

Protein G affinity chromatography

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

F13A1/1448

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml

Storage

Store the Factor XIIIa antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) partial human recombinant protein and 2) human HeLa lysate with Factor XIIIa antibody (clone F13A1/1448). Expected molecular weight ~83 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human histiocytoma with Factor XIIIa antibody (clone F13A1/1448). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Protein array validation of the Factor XIIIa antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Factor XIIIa antibody (clone F13A1/1448). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free Factor XIIIa Antibody (clone F13A1/1448). Confirmation of Integrity and Purity of the Antibody.