Explore
Validate
LearnH00007337-M01
antibody from Abnova Corporation
Targeting: UBE3A
ANCR, AS, E6-AP, EPVE6AP, FLJ26981, HPVE6A
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00007337-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007337-M01, RRID:AB_463912
- Product name
- UBE3A monoclonal antibody (M01), clone 2F6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant UBE3A.
- Antigen sequence
ETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCL
KMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQ
ELLGEERRNKKGPRVDPLETELGVKTLDCR- Isotype
- IgG
- Antibody clone number
- 2F6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references E6 proteins from multiple human betapapillomavirus types degrade Bak and protect keratinocytes from apoptosis after UVB irradiation.
Underbrink MP, Howie HL, Bedard KM, Koop JI, Galloway DA
Journal of virology 2008 Nov;82(21):10408-17
Journal of virology 2008 Nov;82(21):10408-17
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- UBE3A monoclonal antibody (M01), clone 2F6. Western Blot analysis of UBE3A expression in PC-12.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged UBE3A is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to UBE3A on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to UBE3A on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
