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LearnPAB4222
antibody from Abnova Corporation
Targeting: UBE3A
ANCR, AS, E6-AP, EPVE6AP, FLJ26981, HPVE6A
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4222 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4222, RRID:AB_1579947
- Product name
- UBE3A polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of UBE3A.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Solution structure determination and mutational analysis of the papillomavirus E6 interacting peptide of E6AP.
The hPLIC proteins may provide a link between the ubiquitination machinery and the proteasome.
Structure of an E6AP-UbcH7 complex: insights into ubiquitination by the E2-E3 enzyme cascade.
Be X, Hong Y, Wei J, Androphy EJ, Chen JJ, Baleja JD
Biochemistry 2001 Feb 6;40(5):1293-9
Biochemistry 2001 Feb 6;40(5):1293-9
The hPLIC proteins may provide a link between the ubiquitination machinery and the proteasome.
Kleijnen MF, Shih AH, Zhou P, Kumar S, Soccio RE, Kedersha NL, Gill G, Howley PM
Molecular cell 2000 Aug;6(2):409-19
Molecular cell 2000 Aug;6(2):409-19
Structure of an E6AP-UbcH7 complex: insights into ubiquitination by the E2-E3 enzyme cascade.
Huang L, Kinnucan E, Wang G, Beaudenon S, Howley PM, Huibregtse JM, Pavletich NP
Science (New York, N.Y.) 1999 Nov 12;286(5443):1321-6
Science (New York, N.Y.) 1999 Nov 12;286(5443):1321-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The UBE3A polyclonal antibody (Cat # PAB4222) is used in Western blot to detect UBE3A in Y-79 cell lysate .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE3A polyclonal antibody (Cat # PAB4222) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
