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LearnNB500-240
antibody from Novus Biologicals
Targeting: UBE3A
ANCR, AS, E6-AP, EPVE6AP, FLJ26981, HPVE6A
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- NB500-240 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-240, RRID:AB_10002763
- Product name
- Rabbit Polyclonal E6AP/UBE3A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Spatial and temporal silencing of the human maternal UBE3A gene.
Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a.
Adeno-associated virus-mediated rescue of the cognitive defects in a mouse model for Angelman syndrome.
Isoform-specific degradation of PR-B by E6-AP is critical for normal mammary gland development.
Genomic imprinting of experience-dependent cortical plasticity by the ubiquitin ligase gene Ube3a.
Thiothymidine plus low-dose UVA kills hyperproliferative human skin cells independently of their human papilloma virus status.
Deficiency of Rbbp1/Arid4a and Rbbp1l1/Arid4b alters epigenetic modifications and suppresses an imprinting defect in the PWS/AS domain.
A mixed epigenetic/genetic model for oligogenic inheritance of autism with a limited role for UBE3A.
Daily J, Smith AG, Weeber EJ
European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society 2012 Nov;16(6):587-91
European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society 2012 Nov;16(6):587-91
Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a.
Meng L, Person RE, Beaudet AL
Human molecular genetics 2012 Jul 1;21(13):3001-12
Human molecular genetics 2012 Jul 1;21(13):3001-12
Adeno-associated virus-mediated rescue of the cognitive defects in a mouse model for Angelman syndrome.
Daily JL, Nash K, Jinwal U, Golde T, Rogers J, Peters MM, Burdine RD, Dickey C, Banko JL, Weeber EJ
PloS one 2011;6(12):e27221
PloS one 2011;6(12):e27221
Isoform-specific degradation of PR-B by E6-AP is critical for normal mammary gland development.
Ramamoorthy S, Dhananjayan SC, Demayo FJ, Nawaz Z
Molecular endocrinology (Baltimore, Md.) 2010 Nov;24(11):2099-113
Molecular endocrinology (Baltimore, Md.) 2010 Nov;24(11):2099-113
Genomic imprinting of experience-dependent cortical plasticity by the ubiquitin ligase gene Ube3a.
Sato M, Stryker MP
Proceedings of the National Academy of Sciences of the United States of America 2010 Mar 23;107(12):5611-6
Proceedings of the National Academy of Sciences of the United States of America 2010 Mar 23;107(12):5611-6
Thiothymidine plus low-dose UVA kills hyperproliferative human skin cells independently of their human papilloma virus status.
Reelfs O, Xu YZ, Massey A, Karran P, Storey A
Molecular cancer therapeutics 2007 Sep;6(9):2487-95
Molecular cancer therapeutics 2007 Sep;6(9):2487-95
Deficiency of Rbbp1/Arid4a and Rbbp1l1/Arid4b alters epigenetic modifications and suppresses an imprinting defect in the PWS/AS domain.
Wu MY, Tsai TF, Beaudet AL
Genes & development 2006 Oct 15;20(20):2859-70
Genes & development 2006 Oct 15;20(20):2859-70
A mixed epigenetic/genetic model for oligogenic inheritance of autism with a limited role for UBE3A.
Jiang YH, Sahoo T, Michaelis RC, Bercovich D, Bressler J, Kashork CD, Liu Q, Shaffer LG, Schroer RJ, Stockton DW, Spielman RS, Stevenson RE, Beaudet AL
American journal of medical genetics. Part A 2004 Nov 15;131(1):1-10
American journal of medical genetics. Part A 2004 Nov 15;131(1):1-10
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
