M00605

antibody from Boster Biological Technology

Targeting: LEF1 TCF10, TCF1ALPHA, TCF7L3

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00605

Provider

Boster Biological Technology

Product name

Anti-LEF1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for LEF 1/LEF1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Rat LEF 1/LEF1 information: Molecular Weight: 44201 MW; Subcellular Localization: Nucleus . Found in nuclear bodies upon PIASG binding; Tissue Specificity: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.

Reactivity

Human, Rat

Host

Rabbit

Antibody clone number

CFG-12

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human spleen, using LEF1 Antibody(M00605)LEF1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LEF1 Antibody (M00605)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image