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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [4]
- ELISA [1]
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- Product number
- H00051176-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00051176-M01, RRID:AB_540154
- Product name
- Mouse Monoclonal LEF1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. LEF1 - lymphoid enhancer-binding factor 1
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Rac1 augments Wnt signaling by stimulating β-catenin-lymphoid enhancer factor-1 complex assembly independent of β-catenin nuclear import.
Regulation of β-catenin nuclear dynamics by GSK-3β involves a LEF-1 positive feedback loop.
LEF-1 negatively controls interleukin-4 expression through a proximal promoter regulatory element.
Jamieson C, Lui C, Brocardo MG, Martino-Echarri E, Henderson BR
Journal of cell science 2015 Nov 1;128(21):3933-46
Journal of cell science 2015 Nov 1;128(21):3933-46
Regulation of β-catenin nuclear dynamics by GSK-3β involves a LEF-1 positive feedback loop.
Jamieson C, Sharma M, Henderson BR
Traffic (Copenhagen, Denmark) 2011 Aug;12(8):983-99
Traffic (Copenhagen, Denmark) 2011 Aug;12(8):983-99
LEF-1 negatively controls interleukin-4 expression through a proximal promoter regulatory element.
Hebenstreit D, Giaisi M, Treiber MK, Zhang XB, Mi HF, Horejs-Hoeck J, Andersen KG, Krammer PH, Duschl A, Li-Weber M
The Journal of biological chemistry 2008 Aug 15;283(33):22490-7
The Journal of biological chemistry 2008 Aug 15;283(33):22490-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi ( Cat # H00051176-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody (M01), clone 3H5 (Cat # H00051176-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 expression in transfected 293T cell line by LEF1 monoclonal antibody (M01), clone 3H5.Lane 1: LEF1 transfected lysate(44.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5 Analysis of LEF1 expression in MES-SA/Dx5.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5. Analysis of LEF1 expression in HL-60.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: LEF1 Antibody (3H5) [H00051176-M01] - Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a capture antibody.
