H00001073-M03

antibody from Abnova Corporation

Targeting: CFL2 NEM7

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001073-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001073-M03, RRID:AB_518724

Product name

CFL2 monoclonal antibody (M03), clone 6G9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant CFL2.

Antigen sequence

VGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYET
KESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKK
KFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSL
EGKPL


Isotype

IgG

Antibody clone number

6G9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CFL2 monoclonal antibody (M03), clone 6G9 Western Blot analysis of CFL2 expression in HeLa ( Cat # L013V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CFL2 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to CFL2 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to CFL2 on formalin-fixed paraffin-embedded human skeletal muscle. [antibody concentration 8 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol